![Virus cultivation in cell culture](https://kumkoniak.com/71.jpg)
Next, the tissue pieces are subjected to treatment with agents that disrupt the extracellular matrix that holds cells together. Tissues are first dissociated into smaller pieces by mechanical disruption (such as by cutting into smaller pieces using scissors). We define tissue as an aggregate of similar cells forming a definite kind of structural material with a specific function in a multicellular organism.
![virus cultivation in cell culture virus cultivation in cell culture](https://i1.rgstatic.net/publication/342626686_Application_of_an_Inclined_Settler_for_Cell_Culture-Based_Influenza_A_Virus_Production_in_Perfusion_Mode/links/5efdd337299bf18816fa60d5/largepreview.png)
explant culture, the term “cell culture” is used in this review instead of “tissue culture”.Ĭell cultures can be prepared from unicellular cells (e.g., white blood cells) or from a piece of “tissue”. Since the literal meaning of tissue culture is the culturing of tissue pieces, i.e. This review covers only cell culture for animal viruses. The only other practical alternatives to cell culture are to propagate the viruses in susceptible animal or plant hosts. Both animal and plant cells are propagated in cell cultures. Cell culture remains integral with virology, as viruses are obligate intracellular parasites that require replication within a living cell to produce copies of themselves (i.e., to form progeny virions). The term “cell culture” is often used interchangeably with “tissue culture”.
![virus cultivation in cell culture virus cultivation in cell culture](https://c8.alamy.com/comp/BMNBA8/cell-culture-flasks-growing-viruses-in-an-incubator-BMNBA8.jpg)
![virus cultivation in cell culture virus cultivation in cell culture](https://www.onlinebiologynotes.com/wp-content/uploads/2017/09/Batch-Fed-batch.jpg)
In virology, cell culture usually refers to the in vitro growth and manipulation of cells from a tissue obtained from a multicellular organism.
![Virus cultivation in cell culture](https://kumkoniak.com/71.jpg)